Phospho 4ebp1 antibody

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August undefined, 2024

WebPhospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence. WebRabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. 4EBP1, when bound to eIF4E, inhibits cap-dependent ...

Recombinant Anti-eIF4EBP1 (phospho T70) antibody …

Web实验用到的供体是Thr46（铽标记的anti-phospho-4EBP1），受体是绿色荧光标记的4EBP1，是mTOR下游信号通路的蛋白，有活性的mTOR能磷酸化4EBP1，使之与供体结合。如果mTOR活性受到抑制，供体受体距离不能达到荧光能量转移的要求，则在515nm处与485nm处的信号比值增大。 solar screens bullhead city az

Rapamycin differentially inhibits S6Ks and 4E-BP1 to mediate ... - PNAS

WebAll lanes : Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] at 1/1000 dilution Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate Lane 2 : HeLa treated with 100nM rapamycin for 24 hours, whole cell whole cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at … WebThis antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1. Tested applications Suitable for: IHC-P, WB more details Species reactivity Reacts with: Rat, Human Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan Immunogen WebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes solar screens conroe tx

Results for "4ebp1" Abcam: antibodies, proteins, kits...

4EBP1 Antibodies - Novus Biologicals

WebOur 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. Use the list below to choose the 4EBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. WebEach 4EBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. solar screens buckeye azWebPhospho-4EBP1 (Thr37, Thr46) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View all (63) 4EBP1 antibodies Add To Cart Save to list Performance Guarantee Datasheet Protocols Questions & Answers Tech Support Antibody Testing Data (1) solar screens az

"WebSep 7, 2024 · 4E-BP1 Antibody (P-1) is recommended for detection of 4E-BP1 of mouse, rat, human and porcine origin by WB, IP, IF, IHC (P) and ELISA. Anti-4E-BP1 Antibody (P-1) is available conjugated to agarose for IP; HRP for WB, IHC (P) and ELISA; and to either phycoerythrin or FITC for IF, IHC (P) and FCM. " - Phospho 4ebp1 antibody

Phospho 4ebp1 antibody

Anti-4E-BP1 (phospho Ser65 + Thr70) Antibody (A255095)

WebFigure 3: Anti-4E-BP1 (phospho Ser65 + Thr70) Antibody (AF2308) Western blot analysis of extracts from 293 cells treated with 20% FBS , using Phospho-4EBP1(Ser65/Thr70) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide. WebThe Phospho-4EBP1 (Thr37/46) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state.

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WebProduct Description. Rabbit anti 4EBP1 antibody recognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation. The association between 4EPB1 and eukaryotic ... WebListed below are anti-4EBP1 antibodies from multiple suppliers. 4EBP1 is a reported alias name for the human gene EIF4EBP1, or 'eukaryotic translation initiation factor 4E binding protein 1'. ... Immunotag™ Phospho-4EBP1(Ser65/Thr70) Antibody. Applications: WB, IHC; Reactivity: Hu, Ms, Rt; Conjugate/Tag: Unconjugated, Biotin, FITC, HRP, Alexa ...

WebPhospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003). Species … WebMA Beigh's Publication in Biomed Res Int....Sigma unless otherwise indicated. Anti-S6K antibody (Santa Cruz Biotechnologies Inc), Anti-S6K (Polyclonal-Genscript), Anti-Erk1, Anti-phospho-Erk, Anti-4EBP1, Anti-phospho- 4EBP1-T70 and Anti-phospho-S6K Thr412 antibodies were obtained from Cell Signaling...

WebThe Simple Western TM antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. WebDot blot analysis of eIF4EBP1(phospho S65) using ab259329 at 1/1000 (0.522 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Lane 1: eIF4EBP1 (phospho S65) peptide. …

WebAbcam - antibodies and reagents supplier, find any antibody Hello. We're improving abcam.com and we'd welcome your feedback. ... 4ebp1 35 results for 4ebp1 ... Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] - BSA and Azide free (ab283288)

WebAnti-4E-BP1 (phospho Ser65 + Thr70) Antibody (A255095) Overview Specifications Images £240 View Datasheet Rabbit polyclonal antibody to 4E-BP1 (phospho Ser65 + Thr70) for WB, IHC and ICC/IF. 100% Guarantee Price Match Guarantee Product Size: 100µl 200µl Quantity: Add To Cart Shipping Information Contact Us Guarantees Specifications Name sly fox brewery can jamWeb丁香通为您提供磷酸化4e结合蛋白1抗体商品详情介绍：价格：询价，货号：qy-m3780，品牌：乔羽，产地：进口/国产，保存条件 ... solar screens for 4wdWebNov 11, 2008 · To determine whether CDKs could be responsible for the rapamycin-stimulated rephosphorylation of 4EBP1, we treated cells with saturating concentrations of roscovitine (50 μM), a CDK inhibitor. ... Phospho-S6 and actin were blotted for normalization. (D) The experiments were completed in the same way as in C, except WT 4E-BP1, AA 4E … sly fox brewery \u0026 tastin\u0027 room pottstownWebJun 12, 2014 · Total 4EBP1 antibody detects both phosphorylated and non-phosphorylated protein. Samples were treated with lambda-phosphatase or reaction buffer alone and decrease in phosphorylation was noted. X-axis represents iso-electric pH and y-axis represents luminescence units. sly fox brewery jobsWebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … sly fox brewery goat racesWeb肢体畸形相关蛋白FHOD1抗体为客户提供优质的产品服务及最新的产品信息和最优惠的价格，购买时注意检查收货地址，我们不断丰富产品的种类，且全国各网点均可安全、快速配送上门，为您提供超速满意的产品服务，强烈建议广大客户购买前联系我们，确认产品信息及库存状况。肢体畸形相关蛋白 ... solar screens for back porchWeb4EBP1 is a 118 amino acid protein with a predicted molecular weight of 13 kD. Distribution Cytosol and nucleus/ubiquitously expressed Function Regulation of global translation Biology Area Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction Molecular Family Phospho-Proteins Antigen References sly fox brewery locations